Water is of great importance for humans, and for plant and animal life. Today's largescale exploitation and pollution of natural resources has damaged the quality and available quantity of drinking water. The growing industrial production and the exponential growth of the world's population leads to a serious disruption in the economic development and even the existence of the population of certain regions or countries due to limited amounts of drinking water. This final paper presents the results of microbiological, chemical and physical analyzes of water quality from three surface sources in the area of the city of Požega, from which water has been used for drinking since ancient times, namely the sources: Štitnjak, Tekija and Fratrovica. The analyzes were performed at the Institute for Public Health of Požega-Slavonia County. The results of the microbiological analysis of the sampled water from Štitnjak and Fratrovica springs do not correspond to the maximum allowed concentrations. The presence of Enterococcus was determined. The presence of Pseudomonas aeruginosa bacteria was found in all three springs, one colony was found at the Fratrovice spring, while a significant increase in colonies was found at the other two springs. All three samples determined with the presence of aerobic mesophilic bacteria at 22 °C and 36 °C satisfy the maximum allowed concentrations. The results of the chemical and physical analysis of the sampled water indicate that all parameters are in accordance with the maximum allowed concentration.